Characteristics Of Isolates - 1056 Words

In contrast, enterocin TA0033 was unstable at 100 ºC and autoclaving temperatures and lost its activity after exposure to the indicated temperatures. Partial loss in activity was seen with enterocin TA0033 at 60 and 80 ºC after 60min of exposure with 75% reduction in activity within 90 min. In contrast, no loss of activity of enterocin TA102 was seen at these temperatures during the tested time intervals and no significant difference (p0.05) in activity between treated and untreated supernatant were observed. Enterocin TA0033 displayed bactericidal mode of action at 6,400 AU/ml, whereas bacteriostatic activity was detected at lower concentrations (200 AU/ml). Enterocin TA102 demonstrated bacteriostatic mode of action as seen by absence of†¦show more content†¦Discussion Human milk is a potential source of probiotic bacteria, including lactobacilli, streptococci, bifidobacteria and enterococci to the infant gut, affecting the overall composition of the neonate gut microbiota. Although enterococci are widely recognized as probiotic bacteria but opposed to other LAB genus they have yet not been assigned the GRAS (generally recognized as safe) status. E.faecium is considered a suitable probiotic candidate for modulation of immune responses against pathogens (24). In this study, we observed the probiotic properties of the two Enterococcus species including their resistance in stress conditions like acidic environment, high bile salt concentrations, and simulated gastric and intestinal conditions. Another important characteristic of the isolates in study, was their wide antibacterial spectrum against a number of Gram-positive and Gram-negative pathogens. In agreement with our findings, Ghrairi and colleagues (25), reported E. faecium MMT21 bacteriocin ab ility to inhibit not only closely related LAB, but also L. monocytogenes and S. aureus. Different spectrum of inhibitory action may be based on the bacteriocin producing strain, the indicator strain, and also the method used for bacteriocin detection. During physico-chemical characterizations of the antagonistic agent produced by the two Enterococcus species in study, it was observed that the antibacterial actions exertedShow MoreRelatedWhat Are Bacteria And Other Organisms?1458 Words   |  6 Pagestreatment and strategy can be developed to remedy the problem (Intrieri, 2014). In order to identify bacteria and other organisms, scientists have developed many different tests to differentiate microorganisms from each other their physical characteristics. There are many different physiological tests one can used to identify bacteria. For this project, one can use a spore stain to see if a bacteria can produce spores in response to nutrient depletion.(Intrieri, 2014) There is also the catalaseRead MoreMango Essay806 Words   |  4 Pagesanalysis. In the present study, morphological characteristics of the isolates agreed with the description of C. gloeosporioides, but cultural characteristics, which may be diverse due to cultural conditions, and conidial morphology cannot distinguish between species in the C. gloeosporioides complex (Weir et al. 2012). Based on morphological identification, single genes sequence analysis and the five-gene-combined phylogenetic analysis, the isolates obtained from mango in Guangxi included in thisRead MoreBiological Substances And Its Effects On Bacterial Infections1557 Words   |  7 Pagessynthesis as well as contribute to the development of thousands of clinical and medicinal applications. In this experiment, microbial isolates were extracted from various soil samples, purified, and screened through a variety of antibiotic and genomic processes to determine antibiotic attributes. These attributes would be associated with classifying and identifying the isolates down the genus level to be used for further studies and investigations. Materials and Methods: Soil Sample Collection Two soilRead MoreFollowership: Leadership and Followers1218 Words   |  5 PagesThere have been many observations regarding the characteristics of a leader, but followership has received less attention. Treister and Schultz (1997) â€Å"suggests that a follower is someone who accepts guidance and, on receiving it, takes the appropriate action. Treister et al (1997) also state followership is â€Å"considered a passive or submissive role of lesser importance than leadership. However, the effectiveness of leaders to a large measure depends on the qualities of their followers.† TreisterRead MoreStreak Plate Essay654 Words   |  3 PagesWhen an agar plate is inoculated, why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time, propose is to isolate the unknown bacteria. Therefore, the first time to streak on the plate, there are million of bacteria on the loop. For that reason, we need to sterilize the loop before next streaking. Then we can get small group of colonies out from the large groupRead MoreStreptococcus Pneumoniae ( S. Pneumoniae Essay938 Words   |  4 PagesA definitive diagnosis of IPD is provided by the isolation of S. pneumoniae from normally sterile body sites such as blood, cerebrospinal and pleural fluids. However, this is only achievable for a few cases of IPD. This is because the ability to isolate S. pneumoniae from clinical specimens is significantly reduced by prior antibiotic therapy [7]. In the case of pneumococcal pneumonia, the most common clinical manifestation of pneumococcal infections, there are also the added problems of obtainingRead MoreIsol ation And Identification Of Seed Borne Fungi1234 Words   |  5 PagesPrajapati4 1 4 Asst. Professor, College of Agriculture, NAU, Waghai 2. Director of Research Dean PG Studies, NAU, Navsari 3 Asst. Research Scientist, NARP, NAU, Navsari Email:amol_deshmukhnau@yahoo.co.in Abstract An experiment was undertaken to isolate and identify the seed borne fungi of Green gram. Seed samples of three Green gram varieties were collected from Pulse research station, NAU, Navsari. PDA plating method was employed for the isolation of the fungi and cultural, microscopic study wasRead MoreThe Causal Fungus Of Guava Scabby In El Beheira Governorate1099 Words   |  5 Pagessp the Causal Fungus of Guava Scabby Canker in EL-Beheira Governorate, Egypt. ABSTRACT During 2013-2014 growing seasons, forty three isolates of Pestalotiopsis spp were recovered from guava leaves and fruits showed scab symptoms from different regions in EL-Beheira governorate. Five Pestalotiopsis species were recognized according to the morphological characteristics of fungal colony (Colony color, Size and number of acervulii) and conidia (Length, Width, and Color of median cells, Length and theRead MoreCross Sectional Study1531 Words   |  7 Pagescross-sectional study conducted between March 2016 and September 2016, among non-duplicate S. aureus isolates, obtained from clinical isolates and nasal swabs of hospitalized patients and HCWs, in four teaching hospitals (Alzahra, Shariati, Imam Kazem and ShahidChamran) in Isfahan, Iran. Samples collected from different wards, including surgery, intensive care units (ICUs), and internal medicine. Clinical isolates were obtained from the wound, blood, urine culture, sputum, peritoneum and synovial samplesRead MoreEssay Iso lation in Franz Kafka’s The Metamorphosis867 Words   |  4 Pagesthe families’ separation from society. In The Metamorphosis, Kafka emphasizes Gregor’s seclusion from his family. However, Gregor’s separation is involuntary unlike the family who isolates themselves by the choices they make. Each family member has characteristics separating them from society. These characteristics become more unraveling than Gregor, displaying the true isolation contained in The Metamorphosis. Grete’s isolation from society stems from her passion and interest for her